MultiOmics Histology Core Facility
Small intestine sample processed at the HCF with Xenium immune panel, Comet 11-plex panel and an H&E
The MultiOmics Histology Platform aims to be a competence pole providing advanced instrumentation and expert support for the analysis of phenotypes at the tissue level. Built on a collaborative and flexible approach, the facility offers access and expertise to the state of the art multi-omics technologies. Supported by a dedicated and enthusiastic team, the platform is committed to facilitating and accelerating the progress of researchers' projects.
Bridging tissue expertise with cutting-edge multi-omics technologies
The MHCF offers competencies and equipment in histology and performs routine work like sectioning of tissues or classical stains for researchers. It also assists in setting up and optimizing of histological and multi-omics approaches specific for each scientific project, delivering protocols that can be tested either by the researchers themselves, the laboratory technicians, or both. After training, users have free access to the equipments in the core facility, like microtomes or cryostats. Spatial omics technologies such as Comet and Xenium are available on a project-basis. Finally, MHCF makes a large panel of titrated secondary antibodies available to the researchers.
For any questions, please write an email to: [email protected]
Samples Processing
Paraffin Processing
The tissue processor (Sakura VIP6) runs (Overnight routine) from Monday to Thursday at 5PM.
Samples should be in the fridge next to the AI0142 office before 16h30.
We do not accept samples in fixative but only in buffer or 70% Ethanol.
Please rinse tissues with PBS 1x and put them in cassettes.
Place the cassettes in a beaker glass filled with liquid. Name, group and number of cassettes should be written on it.
The cassettes will be available the next day in the paraffin dispenser for making the blocks. Each researcher has to do their own blocks. If you need help, do not hesitate to ask a technician.
For shorter runs (small biopsy, LacZ…), please write an email to [email protected] to plan them.